OrganoReady® BBB HBMEC
OrganoReady® BBB HBMEC is a ready-to-use primary human brain endothelial model that forms perfusable, leak-tight tubules. These tubules provide apical and basolateral access without membrane interference, supporting direct compound exposure and physiologically relevant barrier function. The model is well-suited for therapeutic screening, including toxicity prediction and brain inflammation studies.
Specifications
Each OrganoReady® BBB HBMEC plate comes pre-seeded with 40 or 64 ready-to-use HBMEC tubules grown against validated Collagen-I with stable barriers.
Cell Type: Human brain microvascular endothelial cells (HBMECs)
Format: OrganoPlate® 3-lane 64 or 40
Configuration: HBMEC tubules grown against Collagen-I
Access: Apical and basolateral, membrane-free
Recovery Time: Functional barrier re-forms within a few days post-arrival; no additional seeding or preparation required
Blood-brain-barrier permeability studies
Compound-induced barrier disruption (toxicity)
BBB inflammation
Applications
The OrganoReady BBB HBMEC model provides a physiologically relevant brain microvascular tissue model, suitable for studying key features of BBB function and dynamics. All applications benefit from our OrganoTEER system for barrier function measurement.
Validation
The OrganoReady® BBB HBMEC model demonstrates characteristics of functional and physiologically relevant brain endothelium. The model forms tight and adherent junctions and exhibits active transporter expression, supporting its application in blood–brain barrier research and compound screening.
BBB model shows lumen formation and confirms the 3D architecture of brain endothelial tubules (F-actin)
The blood–brain barrier model shows expression of VE-cadherin, PECAM-1, Claudin-5, and ZO-1, confirming a BBB relevant endothelial phenotype
The HBMEC model demonstrates transporter expression characteristic of the human BBB (GLUT-1, P-gp, BCRP1, MRP-1, TfR)
Latest BBB Webinar
A Perfused Human Blood-Brain Barrier for Disease Modeling and DMPK Studies: This webinar presents 3D human blood-brain barrier models developed in the OrganoPlate® platform. Learn how these models support disease research and drug testing through physiologically relevant barrier function and transport assays.
BBB HBMEC Unboxing Video
How to Access Our OrganoReady® BBB HBMEC
Services
MIMETAS offers customized, fee-for-service solutions using the BBB HBMEC model to support drug development and disease research. The model enables co-culture with immune cells and CNS components, allowing applications in disease modeling. It also supports drug transport studies, including antibody transcytosis and small molecule permeability, making it suitable for therapy optimization, immune modulator development, and safety assessment.
Shipped Product
The OrganoReady® BBB HBMEC is offered as a ready-to-use model shipped directly to your lab, complete with culture media and optimized protocols. After a short recovery, the perfusable tubules are assay-ready, ideal for brain endothelial toxicity and inflammatory studies.