OrganoReady® Angiogenesis HUVEC
OrganoReady® Angiogenesis HUVEC is a ready-to-use primary human vascular model featuring 3D perfusable microvessels. The model includes a HUVEC tubule primed for sprouting, enabling membrane-free formation of angiogenic structures with tip-stalk cell hierarchy and anastomosis. It is well-suited for therapeutic screening to assess modulation of angiogenesis.
Specifications
Each OrganoReady Angiogenesis HUVEC comes pre-seeded with 64 ready-to-use HUVEC tubules ready for sprouting to form perfusable microvessels through a patterned Collagen-I.
Cell type: Human umbilical vein endothelial cells (HUVECs)
Format: OrganoPlate® 3-lane 64
Configuration: HUVEC tubules against Collagen-I, angiogenic sprouting is induced using sprouting mix provided
Access: Apical and basolateral, membrane-free
Recovery Time: HUVEC tubules recover within a few days post-arrival, after which angiogenic sprouting can be initiated; no additional seeding or preparation needed.
Anti-angiogenic compound screening
Vascular toxicity assessment
Pro- and anti-angiogenic response profiling
Applications
The OrganoReady Angiogenesis HUVEC model offers provides a robust vascular tissue platform that supports assays related to angiogenic signaling and compound-induced vascular responses.
Validation
The OrganoReady Angiogenesis HUVEC enables membrane-free sprout formation using primary endothelial cells. Using a defined pro-angiogenic gradient, the model replicates key angiogenic processes, including tip-stalk differentiation and lumen formation.
Validated angiogenic sprouting demonstrated over time highlighting progressive sprout formation following pro-angiogenic factor addition
Show real-time angiogenesis with time-lapse imaging, ideal for mode-of-action studies and compound screening
Compound-induced, concentration-dependent toxicity on angiogenic sprouts confirms the model’s sensitivity to angiogenic disruption
Angiogenesis Whitepaper
High-throughput phenotypic screening with Organ-on-a-chip: a perfused 3D microfluidic angiogenesis assay: This white paper outlines how the OrganoPlate® platform was integrated with automated workflows to perform a 1,546-compound screen using a 3D angiogenic sprouting assay, demonstrating its potential for high-throughput, image-based phenotypic drug screening.
Angiogenesis Model Video
How to Access Our OrganoReady® Angiogenesis HUVEC
Services
MIMETAS offers customized, fee-for-service solutions using the angiogenesis model to accelerate therapy development and target validation. This 3D vascular model supports dynamic sprouting, co-culture, and compound screening, enabling lead development in disease-relevant contexts such as oncology, diabetic retinopathy, and arthritis.
Shipped Product
The OrganoReady® Angiogenesis HUVEC is offered as a ready-to-use model shipped directly to your lab, complete with sprouting mix, culture media and optimized protocols. After initiation of sprout formation, the perfusable microvessels are assay-ready, ideal for vascular toxicity studies and angiogenesis modulation.