• We offer tailored partnerships to support and transform drug discovery.

    Every collaboration is a bespoke arrangement, meticulously designed to discover new compounds and targets in patient relevant disease models.

  • MIMETAS offers flexible fee-for-service solutions for therapy prioritization, optimization, and de-risking of compounds.

    As an extension of your team, MIMETAS provides the expertise and resources needed for effective research and development.

  • Our OrganoReady program is made for you when you need optimized assays for investigational toxicology.

    We guarantee OrganoReady performance according to specifications, in a fast and convenient sales transaction with a clear fee structure.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
open menu icon close menu icon
EN

A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment

Leiden, February 3, 2023 – MIMETAS scientists established a 3D Pancreatic Ductal Adenocarcinoma (PDAC) model, enabling the study of cancer treatment response in PDAC organoids.

Complex and physiologically relevant tumor models are critical for effective drug discovery and development. This is particularly relevant for tumors which lack effective treatments such as PDAC. Characterized by a high-density stroma that makes up to 90% of the tumor volume, PDAC comprises a variety of cells, mainly including cancer-associated fibroblasts, and pancreatic stellate cells (PSCs) as well as extracellular matrix components. In addition to having dense stroma, another hallmark of PDAC is extreme hypoxia. It is therefore essential to recapitulate these oxygen levels seen in PDAC, as well as relevant cell types and other integral conditions, when developing PDAC models.

In this study, MIMETAS researchers developed PDAC organoid-based models that included stromal cells as well as low oxygen tension that emulate that of the tumor core. Using the OrganoPlate® 2-lane, a versatile 3D microfluidic platform, they cultured PDAC-PSCs co-cultures, under normoxic and hypoxic conditions. Here, they found phenotypic and transcriptional changes associated with hypoxia. In addition, they investigated different chemotherapeutic treatment responses of PDAC organoids under various oxygen levels and found that treatment responses were altered under the different oxygen levels.

Taken together, their findings highlight the importance of modulating experimental conditions to include relevant oxygen levels in drug response studies in PDAC, enabling a better understanding of treatment failure as well as opportunities to use Hypoxia-inducible factor (HIF) prolyl hydroxylase (PHD) inhibitors or HIF inhibitors for the treatment of PDAC.


Go to the publication Sign up for our Newsletter

Cookies

May we use cookies?
Hi there! Thanks for visiting our website. We use cookies to keep track of our website statistics to optimize the user experience. We also use cookies for marketing purposes. You can set your preferences by selecting the options below. Terms of Use & Privacy Policy
Accept all
Accept selected
Decline all