Perfused Blood Vessels in the OrganoPlate® 3-lane
Our Organ-on-a-Chip platform, the OrganoPlate®
The OrganoPlate® is a microfluidic 3D cell culture plate, supporting up to 40-96 tissue models on a single plate. The OrganoPlate® is based on the industry-standard 384-well plate for full compatibility with liquid handling- and readout equipment. The plates are versatile, supporting multiple tissue designs, that can be implemented in a straightforward way.
The new 3-lane OrganoPlate® lends itself perfectly for perfused endothelial tubules with apical (inside tube) and basal (outside tube) access.
Models and assays in the OrganoPlate®
We developed a range of assays in the OrganoPlate®, including a unique barrier integrity assay to instantly determine blood vessel leakage in response to challenges. Other assays include immunihistochemistry and viability assays.
- Barrier integrity assay
- Immunohistochemistry with fluorescent detection
- Neuronal activity using calcium imaging
- Cell viability assays
- Other functional assays
Figure: Confocal image animation of co-culture with astrocytes and a perfused HUVEC tubule stained by immunohistochemistry in the OrganoPlate®.
This how the OrganoPlate® works
The OrganoPlate® employs a unique patented liquid handling technology, called Phaseguides™. Phaseguides™ are meticulously designed meniscus pinning barriers. They enable precise, barrier-free definition of culture matrices and cells in 3D, supporting co-culture, cell-cell interactions and unprecedented imaging and quantification. OrganoPlates® are versatile, supporting chemical gradients of chemical compounds and proteins and co-culture conditions. Complex tissue models can be implemented in a straightforward way.
This video demonstrates the working principles of the OrganoPlate®
Membrane-free tissue configurations in the 3-lane OrganoPlate®
In the 3-lane OrganoPlate®, 3 culture lanes can be combined to design tissues. The 3-lane OrganoPlate® supports perfused tubules, perfused cells in ECM-gel and combinations.
The OrganoPlate® supports a range of (co-) culture setups to create complex tissues. This allows detailed studies of cell interactions, strongly enhanced by barrier-free co-culture. Here we show examples of model configurations in the 3-lane OrganoPlate®.