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Perfused gut epithelium tubules

Perfused gut epithelium tubules

3D intestinal tubules in the OrganoPlate®

3D reconstruction of a confocal z-stack showing tubular morphology with a perfused lumen in the 3-lane OrganoPlate®.

The MIMETAS OrganoPlate® is the platform of choice to create physiologically-relevant intestinal epithelial tissues. The OrganoPlate® offers crucial benefits for gut models:

  • Perfused tubular structures without artificial membranes
  • Intestinal leakage and transport studies
  • Healthy and diseased phenotypes
  • Excellent 3D-imaging and easy maintenance
  • Published in Nature Communications
3D reconstruction of a confocal z-stack showing tubular morphology with a perfused lumen in the 3-lane OrganoPlate®. The tube is stained for tight junctions (ZO-1 in red) and brush borders (Ezrin in green.)

Leak-tight intestinal tubules in days

Confocal fluorescence micrograph of a gut tubule in the 3-lane OrganoPlate®, four days after seeding.

The technology of the OrganoPlate® supports the formation of perfused epithelial tubes that mimic intestinal complexity. Epithelial cells are first cultured against the ECM-filled gel channel, after which perfusion of media through the flow channel triggers the formation of a tubular shaped cell layer lining the channel and gel surfaces. Medium flow is crucial to obtain leak-tight epithelial tubules in just a couple of days, ready for use in assays and screens.

Confocal fluorescence micrograph of a gut tubule in the 3-lane OrganoPlate®, four days after seeding. Stained for tight junctions (occludin in red) and microtubules (acetylated tubulin in yellow)

Easily work with the OrganoPlate®, use any number of chips in just 4 steps!

As easy as 2D culture, without pumps and tubules and with perfect imaging assays. The OrganoPlate® is easy to work with. Whether you want to pipet manually for optimal flexibility, or fully automated, you get excellent results. Use as many chips as you need, save the rest for other experiments!

Watch this short (1:42 min) video about working with the OrganoPlate.

Healthy and diseased gut models in any throughput in the 3-lane and 2-lane OrganoPlate®

The 2-lane OrganoPlate®
The 3-lane OrganoPlate®

OrganoPlate®-based intestinal models are membrane-free and closely mimic the in vivo gut epithelium. Healthy gut models in the OrganoPlate® can be applied to study epithelial features and transport mechanisms, or for toxicity tests and compound screenings. Depending on the required level of throughput and on your type of experiment, the 2-lane OrganoPlate® (96 chips) or the 3-lane OrganoPlate® (40 chips) can be used to establish individually-addressable intestinal cultures. Although the OrganoPlate® allows for high-throughput screening of perfused 3D gut tubes, experiments can also be performed on just a few chips.

In addition, the OrganoPlate® allows you to study intestinal epithelial mechanisms in diseased gut models. Caco-2 cells are the gold standard for intestinal models, but it’s also possible to use other cell sources to work with selected genotypes. By using patient-derived iPS cells or primary material, you can test epithelial properties and functionality in diseased conditions, such as inflammatory states.

The next generation of gut transport, disease studies and compound screens in the OrganoPlate® is now within your reach

Gut models in the OrganoPlate® allow you to study transport processes and barrier permeability of the intestinal epithelium. As multiple gut micro-tubules are cultured in parallel on a single plate, their response to pharmacological stimuli can be recorded automatically. It is also possible to assess interactions between compounds and different intestinal transporters by using pharmacological inhibitors of specific transporters. In addition, you can use fluorescently labeled compounds to assay transport across the barrier in real-time. It will soon be possible to co-culture microbiota within these intestinal tubes, to study interaction with the microbiome. The models lend themselves to modelling of inflammatory bowel disease (IBD), Crohn's disease, Irritable Bowel Syndrome (IBS), Ulcerative Colitis (UC), diarrhoea and many other disease applications.

Confocal fluorescence micrograph a seven day old gut tubule, stained for tight junctions (occludin in red), microtubules (acetylated tubulin in yellow)