Technology behind the MIMETAS OrganoPlate

Technology

Technology

This how the OrganoPlate® works

The OrganoPlate® is a microfluidic 3D cell culture plate, supporting up to 96 tissue models on a single plate. Phaseguides™ enable precise, barrier-free definition of culture matrices and cells in 3D, supporting cell-cell interactions and unprecedented imaging and quantification.

This video demonstrates the working principles of the OrganoPlate®

Continuous perfusion without pumps and tubing

Continuous perfusion of media through the microfluidic networks in the OrganoPlate® mimics blood flow and enables exchange of nutrients, oxygen and metabolites. Our unique gravity-driven leveling technology maintains flow without the use of pumps and tubing, making the OrganoPlate® suitable for any throughput. The Perfusion Rocker™ Mini provides continuous medium flow in the OrganoPlate® with minimal settings required.

The principle of gravity-driven, pump-free perfusion in OrganoPlates®

Scalable and robust microfluidic liquid handling

Filling an OrganoPlate® is easy with a standard pipette

The PhaseGuide™ platform is arguably the most cost-effective microfluidic liquid handling platform available. Being a passive technology, no moving parts are required and plate functionality is fully programmed by its geometry. OrganoPlates® make organ-on-a-chip technology affordable and available to non-specialized end-users.

Ultimate design flexibility

The OrganoPlate® is a 384-well plate format, multiplexed 3D culture device

The addition of culture lanes to the microchambers easily increases the complexity of the tissue models in the OrganoPlate®. Patterning additional cell types adjacent to the cell layers allows culturing of complex, non-homogeneous tissues. Application of chemical gradients or exposure to gases is supported. This flexibility is particularly useful for stem cell differentiation and cell motility studies. No other organ-on-a-chip liquid handling platform offers such flexibility combined with ease of handling.

PhaseGuide™ technology

PhaseGuides are meniscus pinning barriers that passively guide fluids in microfludic devices

The OrganoPlate® employs a unique patented liquid handling technology, called Phaseguides™. Phaseguides™ are meticulously designed meniscus pinning barriers. They enable precise, barrier-free definition of culture matrices and cells in 3D, supporting cell-cell interactions and unprecedented imaging and quantification. The OrganoPlate® is versatile, supporting chemical gradients of chemical compounds and proteins and co-culture conditions. Complex tissue models can be implemented in a straightforward way.