Human blood-brain barrier
Blood-brain barrier model in the OrganoPlate®
The MIMETAS OrganoPlate® is the platform of choice to model the blood-brain barrier. OrganoPlate®- based BBB models offer these unique benefits:
- Membrane-free endothelial barrier tissue culture
- Pump-free perfused microcapillaries
- BBB transport and leakage studies
- Healthy and diseased phenotypes
- Excellent 3D-imaging and easy maintenance
Figure: Confocal image animation of a co-culture with primairy astrocytes and HUVECs.
Blood-brain-barrier models in the OrganoPlate®
The two-lane OrganoPlate® supports the formation of microvessels, which are in direct contact with astrocytes in the adjacent lane. To create a blood-brain barrier model, you can seed human astrocytes in the gel channel of the OrganoPlate®. After gelation, you can seed endothelial cells in the medium channel. Supported by perfusion, endothelial cells form a microvessel against the ECM gel. Astrocytes can now migrate towards the endothelial cells to support the vessel. The result is a membrane-free endothelial barrier tissue at the interface between the two lanes. Within a week, the BBB model is ready for use in assays.
Figure: Co-culture with astrocytes and HUVECs in the OrganoPlate®.
BBB models in any throughput
OrganoPlate®-based blood-brain barrier models are membrane-free and closely mimic the in vivo BBB. This makes the model ideal to study BBB features and mechanisms. BBB models in the OrganoPlate® can also be applied for toxicity screenings. In the two-lane OrganoPlate®, you can test toxic effects of drug candidates on 96 BBB models per individual OrganoPlate®.
In addition, the OrganoPlate® allows you to study blood-brain barrier integrity in healthy and diseased tissues. By using patient-derived iPS cells or primary material, you can test BBB properties and functionality in diseased conditions, such as neurodegenerative or inflammatory states. It also allows you to screen for drug candidates in disease-affected BBB models.
BBB transport studies in the OrganoPlate® are within reach
The direct interaction between endothelial cells and astrocytes in the OrganoPlate® mimics the semi-permeable barrier function of the blood-brain barrier. It renders BBB models in the OrganoPlate® highly suitable to study transport processes and barrier permeability. You can assess BBB transport by comparing medium content from the lumen (apical) and parenchyma (basal) sides of the barrier tissue with ELISA, mass spectrometry or other assays. In addition, it is possible to assess interactions between compounds and different BBB transporters by using pharmacological inhibitors of specific transporters. You can also use fluorescently labeled compounds to assay transport across the barrier in real-time.