Getting started & OrganoPlate® Protocols | Mimetas

Getting started & OrganoPlate® Protocols

Getting started & OrganoPlate® Protocols

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We want to offer you the best possible experience when using the OrganoPlate® for your experiments. This page contains training videos and protocols to get you started. For optimal user experience, we recommend you to follow these 5 steps before your first experiment:

  1. Watch the training video(s) below that fit your model of choice (in-gel, tubule or co-culture)
  2. Read the basic protocol (for required materials and crucial steps)
  3. Practice loading the gel channel of the OrganoPlate® with 2μL distilled water (first column A1-P1; 16 culture units)
  4. Prepare buffers, stock solutions, cells, and adjust the protocol to your experimental needs (if required)
  5. Start using the OrganoPlate® for your experiments

We hope these steps provided sufficient information to get you started with the OrganoPlate®. If you have any questions or any tips for improvements, don't hesitate to contact us.

Tubule seeding training video

An introduction to seeding tubules in the OrganoPlate®. Watch this trainings video (9 min) to get familiar with seeding cells against an ECM-gel for establishing:

  • Blood vessels
  • Kidney tubules
  • Gut tubules

For establishing co-cultures with tubule- and in-gel seeding, we recommend watching the in-gel training video as well.

 

The in-gel training video

An introduction to in-gel seeding in the OrganoPlate®. Watch this video (8 min) if you want to seed your cells embedded in the ECM-gel, recommended for:

  • CNS (Neurons& glia)
  • Liver (Hepatocytes)

For co-cultures with tubule- and in-gel seeding, we recommend watching the tubule training video as well.