The OrganoPlate
Our Organ-on-a-Chip platform, the OrganoPlate®
The OrganoPlate® is a microfluidic 3D cell culture plate, supporting up to 96 tissue models on a single plate. The OrganoPlate® is based on the industry-standard 384-well plate for full compatibility with liquid handling- and readout equipment. The plates are versatile, supporting multiple tissue designs, that can be implemented in a straightforward way.
3D intestinal tubules in the OrganoPlate®
The MIMETAS OrganoPlate® is the platform of choice to create physiologically-relevant intestinal epithelial tissues. The OrganoPlate® offers crucial benefits for gut models:
- Perfused tubular structures without artificial membranes
- Intestinal leakage and transport studies
- Healthy and diseased phenotypes
- Excellent 3D-imaging and easy maintenance
- Published in Nature Communications
3D reconstruction of a confocal z-stack showing tubular morphology with a perfused lumen in the 3-lane OrganoPlate®. The tube is stained for tight junctions (ZO-1 in red) and brush borders (Ezrin in green.)
Further models in the OrganoPlate®
The MIMETAS OrganoPlate® is an excellent platform for the development of organ models, such as blood vessels, liver, gut, kidney and brain tissues. Next to the organ models, the OrganoPlate® allows co-cultures, for example endothelial cells, neurons and astrocytes to create a blood-brain barrier or endothelial cells and tumor cells to create tumor tissue models. The possibilities are endless, try the OrganoPlate® to see where your imagination takes you.
Validated assays in the OrganoPlate®
- Barrier integrity assay
- Immunohistochemistry with fluorescent detection
- Neuronal activity using calcium imaging
- Cell viability assays
- Other functional assays
Figure: Confocal image animation of co-culture with astrocytes and HUVECs stained by Immunohistochemistry in the OrganoPlate®.
This how the OrganoPlate® works
The OrganoPlate® employs a unique patented liquid handling technology, called Phaseguides™. Phaseguides™ are meticulously designed meniscus pinning barriers. They enable precise, barrier-free definition of culture matrices and cells in 3D, supporting cell-cell interactions and unprecedented imaging and quantification. OrganoPlates® are versatile, supporting chemical gradients of chemical compounds and proteins and co-culture conditions. Complex tissue models can be implemented in a straightforward way.
This video demonstrates the working principles of the OrganoPlate®