Model design in the OrganoPlate
Perfused tissues and tubules in the OrganoPlate®
The OrganoPlate® is a microfluidic 3D culture plate, supporting up to 96 tissue models on a single plate. In an OrganoPlate®, you can grow tissues embedded in an ECM gel or as a perfused tubule against the ECM gel.
In-gel 3D tissue culture
For in-gel culture, cells are mixed with an ECM such as Matrigel® and seeded in the gel inlet of an OrganoPlate®. As soon as the ECM-gel has set, you can add medium to the inlet and outlet wells to perfuse the tissue. This in-gel culturing technique works perfectly for for example tumors, hepatocytes and neuronal cultures, but also for supporting cells types, such as astrocytes, pericytes and podocytes. For in-gel cultures, the type and density of ECM gel are important as these factors influence the growth and properties of tissues (e.g. hepatocytes form smooth spheroids while neurons form neuronal networks in the gel).
Perfused 3D tissue in ECM gel. A 3D representation of a 2-lane OrganoPlate® culture chamber with perfused medium channel, Phaseguide™ (ph) and tissue channel with cells in extracellular matrix gel (ECM).
Against-gel perfused 3D tubules culture
In addition to in-gel cultures, you can deposit monolayers at the interface between lanes. This is achieved by seeding cells against the ECM gel. Supported by perfusion, the cells grow against the gel and the inner wall of the medium channel to form a perfused vessel structure. Examples are a renal proximal tubules, intestinal tubules and endothelial tubule or blood vessels, all grown as a tubule against ECM gels.
Perfused tubule against ECM gel. A 3D representation of a 2-lane OrganoPlate® culture chamber with a perfused epithelial tubule in the medium channel, Phaseguide™ (ph) and gel channel with extracellular matrix gel (ECM).
Tissue configurations in the 2-lane OrganoPlate®
In the 2-lane OrganoPlate®, 2 tissue lanes can be combined to design tissue setups. The two-lane OrganoPlate® supports perfused epithelial tubules and blood vessels, perfused cells in ECM-gel or combinations of both.
The OrganoPlate® supports a range of (co-) culture setups to create complex tissues. This allows detailed studies of cell interactions, strongly enhanced by barrier-free co-culture. Here we show examples of model configurations in the 2-lane OrganoPlate®.
Tissue configurations in the 3-lane OrganoPlate®
In the 3-lane OrganoPlate®, 3 tissue lanes can be combined to design more complex tissue setups, such as two tubules, flanking a central ECM-gel with cells or two adjacent ECM-gels with cells and a perfusion lane. The three-lane OrganoPlate® allows basal and apical access to epithelial tubules and blood vessels, combinations of tissues growing in ECM and tubules, as well as more advanced transport studies.
The three-lane OrganoPlate® supports a range of sophisticated tissue configurations.