The vasculature system plays a critical role in inflammation processes in the body. Vascular inflammatory mechanisms are characterized by increased immune cell recruitment and migration, as well as disruption of blood vessel wall permeability. It is important to develop models that fully replicate changes in vascular barrier permeability in response to inflammatory conditions.
In this poster, we demonstrate a platform for modelling endothelial inflammation in response to a cocktail of inflammatory cytokines and immune cell interaction using 64 perfused microfluidic HUVEC tubules. Using the OrganoTEER®, high-throughput trans epithelial electrical resistance (TEER) measurements were performed to assess barrier function. Additionally, the impact of immune cells on vascular inflammation was examined after adding unstimulated and stimulated Peripheral Blood Mononuclear Cells (PBMCs) to the endothelial tubules.
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