The transition from 2D to 3D cell culture is needed to develop more physiologically more relevant in vitro models for human (disease) modeling and preclinical drug development. Intestinal organoids-on-a-chip are suitable for high-throughput screeninig of compound effects through real time imaging or transport and barrier integrity. Investigations of complex gut-on-chip models enable mimicking disease phenotypes such as inflammatory bowel disease (IBD) and furthermore allow standardized screening to find potential drug targets.
In this poster, we present how we utilized the OrganoPlate® to study physiology and disease mechanisms in patient specific gut models by using adult human stem cell derived intestinal organoids with the microfluidic technology. Our established model indicates rapid cell polarization, expression of intestinal markers and tight junction formtion.
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