Snakebites pose a significant global health threat, affecting millions and causing tens of thousands of fatalities annually. Many snake venoms disrupt blood vessels, leading to severe physiological consequences. To address the need for advanced in vitro models for snakebite research, we used OrganoReady Blood Vessel models to assess snake venom effects on human tissue. This method leverages 3D human blood vessels in a microfluidic system, the OrganoPlate®, enabling precise control over physiological parameters.
In this app note, we describe an assay to assess vascular toxicity from various snake venoms in the OrganoReady® Blood Vessel HUVEC 3-lane 64 using a multimode microplate reader. Image-based protocols monitor endothelial barrier function, cell viability, and morphological changes in real-time with high temporal resolution. The MIMETAS OrganoReady Blood Vessel model with functional readouts provides a valuable tool for investigating endothelial mechanisms efficiently.
Benefits:
- 64 ready-to-use 3D human blood vessels in a perfused microfluidic platform
- High-throughput assessment of vascular inflammation and toxicity
- Investigation of multimodal endothelial toxicity mechanisms using multiplexed assays
Download the app note here
