Customers speak: Jelle Vriend
Today we interview Jelle Vriend, who is a development scientist at Newcells Biotech. During his PhD at the department of Pharmacology and Toxicology at Radboud University Medical Center (Nijmegen, the Netherlands) he has worked with MIMETAS OrganoPlate®. He published two papers as first author on the development of kidney proximal tubules (webinar). We ask him several questions on his experience with the OrganoPlate, his scientific accomplishments and his most treasured quote.
How did the OrganoPlate transform the work you do?
The OrganoPlate® allowed us to culture our human kidney cell line in an in vivo-like environment, by incorporating a 3D tubule-like structure and extracellular matrix. Furthermore, we implemented the Perfusion Rocker Mini™ to study different strengths of fluid shear stress in our model. We could just simply change the settings of the Perfusion Rocker Mini, allowing us to work without the use of pumps to induce different strengths in fluid stress. Thanks to the help of MIMETAS, we knew what settings we had to use to apply the fluid shears we wanted to study.
What did you like most about the OrganoPlate? What could be improved?
The OrganoPlate was compatible with all of our equipment (plate readers, microscopes etc.), easy to use, and the costs of implementation of the OrganoPlate were therefore low. Due to slight batch differences of Collagen I, which was the main component of the ECM we used, the loading of the gel sometimes would be a struggle. This could be improved by sharing information on good batches of Collagen I or new gels which are compatible with the OrganoPlate.
Do you refer the OrganoPlate to others, and if so, why?
Yes, because of its compatibility, flexibility (due to many different organs can be modelled in this platform), and excellent help from Mimetas when setting up assays or protocols with plate readers and microscopes.
Was it easy to implement the OrganoPlate in your workflow?
The OrganoPlate was easy to implement in my workflow. You get used to the plate format fast and no major changes had to be made on current experimental protocols.
How did you get your academic career started and what is so interesting about doing research?
I enjoyed the versatility of working in a lab during my internship in my Master’s program. When I was presenting my work from this internship at an international conference, I found out about the latest developments in 3D organ modelling and got intrigued by this subject. I was lucky enough to find a PhD project in 3D kidney modelling in an international consortium and in collaboration with pharmaceutical companies.
What project would you consider your most significant career accomplishment to date?
To have published two interesting papers using the OrganoPlate and to have been part of the NC3Rs NephroTube consortium where we together have successfully completed the challenge.
What is your favorite quote, song or belief, anything that you live by?
It is an inspiring quote from Winston Churchill: “Success consists of going from failure to failure without loss of enthusiasm.”