Perfusable 3D vasculature
Using Mimetas Organoplates®, we have developed unique 3D vascular models that recapitulate hallmark features of human microvessels. Our vascular models are grown by seeding endothelial cells against collagen ECM under perfusion to generate complete 3D microvessels within the perfusion channel. Some of the unique features offered by our vascular model are described below.
The page banners shows a 3D reconstruction of a perfusable 3D microvessel of HUVEC cells seeded against a collagen I hydrogel.
Under in vivo conditions, vasculature is continuously exposed to shear stress. We incorporate and control the shear stress in our platform to create more physiologically relevant microvessels. The endothelial cells align with the flow direction and attain a quiescent phenotype. The vasculature can be perfused with immune cells, e.g. monocytes, to study adherence at and transmigration.
Cells that normally surround the vasculature, such as pericytes and vascular smooth muscle cells, can be incorporated to generate microvessels with unique physiological characteristics. Interactions between endothelial cells and mural cells, such as the influence of pericytes on barrier function, can be closely monitored.
Precise control over the extracellular matrix allows us to generate gradients. Gradients play a crucial role in angiogenesis, the formation of new blood vessels. After stimulating the microvessel with angiogenic factors, tip cells with their characteristic filopodia invade the extracellular matrix. These are followed by stalk cells with apical-basal polarity and lumen formation. The lumen eventually connects back to the microvessel.