3D neuronal tissues
3D neuronal tissues in the OrganoPlate®
The MIMETAS OrganoPlate® is the platform of choice to create physiologically-relevant neuronal tissues. The OrganoPlate® offers crucial benefits for neuronal models:
- Active, 3D neuronal networks in days
- A range of co-culture options
- Long-term culture and exposure up to months
- Healthy and diseased phenotypes
- Excellent 3D-imaging and easy maintenance
Figure: Confocal image animation of 3D neuronal networks within 24 hours after seeding in the OrganoPlate®. Stained with β3-tubulin (green) and DraQ5 (blue) to visualize neurites and nuclei. Scale bar: 100μm
Active neuronal 3D models in days
A two-lane OrganoPlate® harbors 96 individually-addressable neuronal cultures. In just a couple of days, you can obtain active neuronal networks, by seeding mature iPSC-derived neurons and glia. Alternatively, you can differentiate neuronal stem cells or neural progenitor cells into mature neurons in 4-8 weeks in the OrganoPlate®. iPSC-derived neurons allow you to work with selected, specific genotypes.
We have shown successful differentiation and culture of a range of human brain cell types in the OrganoPlate®, such as astrocytes, glutamatergic, GABAergic and dopaminergic neurons.
Figure: Calcium image recording of a neuronal culture after 6 days in the OrganoPlate®.
Mimicking the physiology of the brain
In the OrganoPlate®, iPSC-derived neurons in combination with adjacent or mixed co-culture techniques allow for the generation of physiologically-relevant brain models. For example, you can co-culture astrocytes and neurons, or neurons with perfused microcapillaries to establish complex structures such as the blood-brain-barrier.
Neuronal models in any throughput
In biology, you often need to test many samples and conditions. 96 tissues on a single OrganoPlate® allow you to assay models in any throughput, rendering neuronal cultures truly screenable. For example, you can evaluate compound libraries in functional 3D neuronal networks by using automated neuronal activity assays. Neurotoxicity can be measured using standardized neurite outgrowth and cell viability assays. Published protocols are available for 3D networks of neurons in the OrganoPlate®.
The future is now
iPSC technology allows you to generate neurons with any genetic background, for example related to neurological diseases. Culturing iPSC-derived neurons in the OrganoPlate® offers the exciting opportunity to develop screenable cell-based models for CNS diseases for fundamental research and compound evaluations.